Validation and recalibration of sex estimation methods using pubic nonmetric traits for the Chilean population.

Chile had a violent military coup (1973-1990) that resulted in 3,000 victims declared detained, missing or killed; many are still missing and unidentified. Currently, the Human Rights Unit of the Forensic Medical Service in Chile applies globally recognised forensic anthropological approaches, but many of these methods have not been validated in a Chilean sample. As current research has demonstrated population-specificity with extant methods, the present study aims to validate sex estimation methods in a Chilean population and thereafter establish population-specific equations. A sample of 265 os coxae of known age and sex of adult Chileans from the Santiago Subactual Osteology Collection were analysed. Visual assessment and scoring of the pelvic traits were performed in accordance with the Phenice (1969) and Klales et al. (2012) methods. The accuracy of Phenice (1969) in the Chilean sample was 96.98%, with a sex bias of 7.68%. Klales et al. (2012) achieved 87.17% accuracy with a sex bias of -15.39%. Although both methods showed acceptable classification accuracy, the associated sex bias values are unacceptable in forensic practice. Therefore, six univariate and eight multivariate predictive models were formulated for the Chilean population. The most accurate univariate model was the ventral arc at 96.6%, with a sex bias of 5.2%. Classification accuracy using all traits was 97.0%, with a sex bias of 7.7%. This study provides Chilean practitioners a population-specific morphoscopic standard with associated classification probabilities acceptable to accomplish legal admissibility requirements in human rights and criminal cases specific to the second half of the 20th century.

Validating the use of clinical MSCT scans for cranial nonmetric sex estimation in a contemporary Indonesian population.

There is renewed interest in Asia for the development of forensic anthropological standards, partly due to the need for population-specific models to maintain high classification accuracies. At present, there are no known studies utilising morphoscopic standards specific to the Indonesian population. Craniometric analyses can often be time-consuming; morphoscopic assessments are quicker and are also known to be reliable and accurate. One of the most utilised morphoscopic standards for the estimation of skeletal sex is that of Walker (2008). Its application across population groups demonstrated reduced accuracies outside of the United States; population-specific predictive models would thus serve to improve the identification process of unknown skeletal remains. Digital imaging also allows for the validation of standards on a contemporary population and is an appropriate proxy to physical skeletal material.The present study quantifies the applicability of the Walker standard to a contemporary Indonesian population. A total of 200 cranial MSCT scans from a hospital in Makassar were analysed using OsiriX®. Scoring was performed in accordance with the Walker standard. Five univariate and nine multivariate predictive models were derived using single trait and multi-trait combinations. The best performing univariate model included the glabella, with a total classification accuracy of 82.0% and a sex bias of 14.6%. Classification accuracy with all traits considered was at 95.2% for females and 82.8% for males with a sex bias of 12.5%. These results provide forensic practitioners in Indonesia with an appropriate morphoscopic sex estimation standard, strengthening their capabilities in the field and improving judicial outcomes.

Age assessment in unaccompanied minors: assessing uniformity of protocols across Europe.

Age assessment of migrants is crucial, particularly for unaccompanied foreign minors, a population facing legal, social, and humanitarian challenges. Despite existing guidelines, there is no unified protocol in Europe for age assessment.The Forensic Anthropology Society of Europe (FASE) conducted a comprehensive questionnaire to understand age estimation practices in Europe. The questionnaire had sections focusing on the professional background of respondents, annual assessment numbers, requesting parties and reasons, types of examinations conducted (e.g., physical, radiological), followed protocols, age estimation methods, and questions on how age estimates are reported.The questionnaire's findings reveal extensive engagement of the forensic community in age assessment in the living, emphasizing multidisciplinary approaches. However, there seems to be an incomplete appreciation of AGFAD guidelines. Commonalities exist in examination methodologies and imaging tests. However, discrepancies emerged among respondents regarding sexual maturity assessment and reporting assessment results. Given the increasing importance of age assessment, especially for migrant child protection, the study stresses the need for a unified protocol across European countries. This can only be achieved if EU Member States wholeheartedly embrace the fundamental principles outlined in EU Directives and conduct medical age assessments aligned with recognized standards such as the AGFAD guidelines.

The influence of age on greater sciatic notch morphology: testing the Walker method in an Australian population.

Sex estimation is an integral aspect of a forensic biological profile. The pelvis, being the most dimorphic part of the skeleton, has been studied in considerable detail relative to morphological and metric variation. However, empirical data on the effect of age on pelvic morphology relative to sex-specific morphological variation is limited, especially in regard to the estimation of skeletal sex. This study assesses whether there are age-related differences in the distribution of the Walker (2005) morphological scores for the greater sciatic notch (GSN) in an Australian population. Three-dimensional volumetric reconstructions derived from multi-detector computed tomography (MDCT) scans of 567 pelves of 258 females and 309 males aged 18 to 96 years were scored following Walker (2005). Differences in score distributions and means by sex and age group were tested using Pearson's chi-squared test and ANOVA, respectively. The accuracy of sex estimates derived from logistic regression equations was explored using leave-one-out cross-validation. Significant differences were found in score distribution and means among age groups in females, but not in males. There was a tendency toward higher scores in older females. The overall sex estimation accuracy was 87.5%. When comparing age groups 18-49 and 70 + years, estimation accuracy decreased in females (99% vs. 91%), while the opposite was found for males (79% vs. 87%). These findings suggest that age affects GSN morphology. Higher mean scores in older females imply that, on average, the GSN becomes narrower with increasing age. It is thus recommended due consideration of estimated age when assessing sex based on the GSN in unidentified human remains.

Demonstrating the empirical effect of population specificity of anthropological standards in a contemporary Australian population.

The ability to differentiate individuals based on their biological sex is essential for the creation of an accurate anthropological assessment; it is therefore crucial that the standards that facilitate this are likewise accurate. Given the relative paucity of population-specific anthropological standards formulated specifically for application in the contemporary Australian population, forensic anthropological assessments have historically relied on the application of established methods developed using population geographically and/or temporally disparate. The aim of the present paper is, therefore, to assess the accuracy and reliability of established cranial sex estimation methods, developed from geographically distinct populations, as applied to the contemporary Australian population. Comparison between the original stated accuracy and sex bias values (where applicable) and those achieved after application to the Australian population provides insight into the importance of having anthropological standards optimised for application in specific jurisdictions. The sample analysed comprised computed tomographic (CT) cranial scans of 771 (385 female and 386 male) individuals collected from five Australian states/territories. Cranial CT scans were visualised as three-dimensional volume-rendered reconstructions using OsiriX®. On each cranium, 76 cranial landmarks were acquired, and 36 linear inter-landmark measurements were calculated using MorphDB. A total of 35 predictive models taken from Giles and Elliot (1963), Iscan et al. (1995), Ogawa et al. (2013), Steyn and Iscan (1998) and Kranioti et al. (2008) were tested. Application to the Australian population resulted in an average decrease in accuracy of 21.2%, with an associated sex bias range between - 64.0 and 99.7% (average sex bias value of 29.6%), relative to the original studies. The present investigation has highlighted the inherent inaccuracies of applying models derived from geographically and/or temporally disparate populations. It is, therefore, imperative that statistical models developed from a population consistent with the decedent be used for the estimation of sex in forensic casework.

Sex estimation of frontal sinus volume from computed tomography scans in a Western Australian adult population.

With the increasing use of radiological three-dimensional imaging, different metric and morphological aspects of the frontal sinuses (FS) have been examined relative to their potential to aid human identification, including sex estimation. The aim of this study is to assess the validity of the metric analysis of the FS volume for estimating sex in a Western Australian (WA) adult population, following the Michel et al. (2015) method. The right, left and total frontal sinus volume (TFSV) from 99 computed tomography scans (47 males and 52 females) of WA individuals aged 18-40 years were three-dimensionally reconstructed using OsiriX® and their volumes acquired in mm3. Sexual dimorphism in the TFSV was tested, and the accuracy of the Michel et al. (2015) formulae was calculated. Population-specific regression equations were also produced. There was a significant difference between the mean TFSV in males and females. The classification accuracy derived from the Michel et al. (2015) formulae was 59.6%. The WA-specific logistic regression analysis yielded an accuracy of 57.2%. The low accuracy rates preclude the use of the sex estimation method in the WA population, but the volume analysis confirmed the individual variability of the frontal sinuses, which may be useful for personal identification.

Stable Isotope Provenance of Unidentified Deceased Migrants-A Pilot Study.

In the global migration crisis, one of the challenges in the effort to identify deceased migrants is establishing their region of origin, which facilitates the search for ante mortem data to be compared with the post mortem information. This pilot study explores the potential of using stable isotope analysis to distinguish between individuals coming from West Africa and the Horn of Africa. Six individuals (four of known origin and two of unknown origin) were sampled. δ13CVPDB(keratin), δ15NVPDB(keratin) and δ18OVSMOW(keratin) of hair were analysed using Elemental Analyzers coupled with Isotope Ratio Mass Spectrometry (IRMS). δ18OVSMOW(carbonate) and δ13CVPDB(carbonate) of bone were analysed using GasBench II with IRMS, while 87Sr/86Sr composition was determined in bone and dental enamel using laser ablation multi-collector inductively coupled plasma mass spectrometry. The stable isotope compositions of the individual from the Horn of Africa differed from the other individuals. The differences found between 87Sr/86Sr of enamel and bone and between δ18O and δ13C in bone and hair reflect changes in sources of food and water in accordance with regionally typical migration journeys. The analysis of multiple stable isotopes delivered promising results, allowing us to narrow down the region of origin of deceased migrants and corroborate the information about the migration journey.

Awareness of forensic anthropology in Switzerland: a survey among forensic practitioners, police, and prosecutors.

Forensic anthropology (FA) as a specialized discipline has been practised in multi-lingual Switzerland for over a decade. A variety of expertise regarding osteological assessments as well as facial image comparison (FIC) is provided by different centres. Nevertheless, information is lacking about the awareness of FA and its benefits for forensic investigations among forensic stakeholders. Therefore, a survey was sent to Swiss anthropologists (AN) and related professions (police officers, prosecutors, and forensic pathologists) to assess three main aspects: (1) the experience of working (biological/forensic) anthropologists within FA; (2) how FA is perceived by other professions within the legal system; and (3) identify gaps (if any) in understanding of FA with the aim to suggest avenues for improvement if necessary. The results show that awareness of FA varies by occupation and cantonal regions. In areas where close collaborations between forensic anthropologists (FAs) and other stakeholders have been formally established, be it with focus on osteological analyses or FIC, the awareness of FA competencies was superior to areas where this was not the case. An overwhelming majority of forensic actors expressed interest in continuing education related to the role of FA. These findings indicate that facilitation of communication and collaboration leads to improvement in the awareness of the competencies of FAs and their contribution to forensic investigations.

Compulsory treatment order and rehospitalisation: A New Zealand study.

OBJECTIVE: The effectiveness of compulsory treatment orders (CTO) in psychiatric practice is an area in need of evidence. There are no recent New Zealand publications on outcomes for patients under CTOs. This study examined the association between CTOs and subsequent rehospitalisation for patients with schizophrenia or related disorders. METHOD: Two year outcome data for 326 consecutive patients discharged in 2013 and 2014 was obtained from the Programme for the Integration of Mental Health Data database. Regression analyses were performed with rehospitalisation as the main outcome. RESULTS: For the 54% of patients discharged under CTOs, rehospitalisation was 2-4 times more likely for the CTO group than for voluntary patients. Patients under CTOs also spent longer in hospital post index admission (IA). However, patients placed under CTOs during IA stayed longer than those under CTOs prior to IA. Ethnicity did not contribute significantly to any of the findings. CONCLUSION: This study did not show that patients under CTOs were associated with subsequent reduced resource use. The subgroup analysis suggested that studies with a longer follow-up period may provide better insight into the utility of CTOs.

The problem of dating fractures: A retrospective observational study of radiologic features of fracture healing in adults.

Accurate dating of bone fractures can be crucial in the context of forensic trauma analysis since it may provide essential information for the corroboration or contradiction of statements by victims or suspects in cases of domestic abuse or torture. The different stages of bone healing have been well described in the existing literature, and some previous studies attempted to define related timelines particularly in the paediatric population. However, the bone healing process can be very variable and despite the importance of the topic in the forensic field, so far little is known about the radiological appearance of bone fractures at different healing stages, and how this correlates with time, especially in an adult population. The aim of this retrospective observational study was to describe the temporal aspect of pre-defined healing stages as they appear on radiographs of tubular bone fractures in adults, and explore the effect of potential cofounding variables. A dynamic nomogram was developed as a user-friendly tool to be eventually applied in clinical or medico-legal settings. This study showed that the posttraumatic time interval (PTTI) increased progressively with the pre-defined healing stages. However, confounding factors, such as patients' age, sex, and location of the fracture need to be accounted for in the final estimation model. Further studies are needed to explore more potentially confounding variables to refine the presented outcomes. Better knowledge of the effect of different confounding variables in the dating of fracture healing will contribute to greater accuracy of PTTI estimation of bone fractures in adults.

Technical Note: The Forensic Anthropology Society of Europe (FASE) Map of Identified Osteological Collections.

Identified (documented) osteological collections represent an important resource in the development of forensic anthropology standards and methods as well as a precious tool for learning and training of practitioners. Even though the number of papers presenting identified collections worldwide increases, many of the collections have still not been divulged to the scientific community in sufficient detail to ascertain their exact number. The Forensic Anthropology Society of Europe (FASE) therefore developed a tool that goes beyond sporadic publications: the FASE Map of Identified Osteological Collections, which is freely accessible and continuously updated and revised. The online map is available at http://forensicanthropology.eu/osteological-collections/. The map of skeletal collections was created in 2017 and currently displays information on 153 identified osteological collections (43 of them categorized as contemporary) located in 41 different countries. This article offers a short analysis of the type, geographical location and content of the collections included in the map. The aim of this article and the map as such is to provide a useful resource to facilitate research planning and teaching in forensic anthropology and related disciplines.

Sociodemographic and clinical variables associated with discharge under compulsory treatment orders.

OBJECTIVE: To document the sociodemographic and clinical variables associated with discharge under compulsory treatment orders in patients with schizophrenia or related disorders. METHOD: Information on various sociodemographic and clinical variables were extracted from the clinical files of 349 patients discharged from an adult (age 18-65) inpatient psychiatric unit with diagnoses of schizophrenia or related disorders. Univariate and multiple logistic regression analyses with legal compulsion (compulsory versus voluntary) as outcomes were applied. RESULTS: Two hundred of the 349 discharged patients were placed under compulsory treatment orders. In the univariate logistic regression analysis, unemployment, diagnosis of schizophrenia, forensic history, dosage of antipsychotics, prescription and frequency of injectable antipsychotics, polypharmacy and a hospital stay of more than 28 days were associated with compulsory status. Being married or in partnership and living with partner was associated with voluntary status. Multiple regression models respectively confirmed most of these relationships observed in the univariate analyses. CONCLUSION: Clarification of the apparent drivers for compulsory treatment may help thoughtful reductions in the use of compulsion.

Strengthening the role of forensic anthropology in personal identification: Position statement by the Board of the Forensic Anthropology Society of Europe (FASE).

In this position statement, the Board members of the Forensic Anthropology Society of Europe (FASE) argue that forensic anthropology methods can be used as means of personal identification, particularly in situations with limited availability of traditional identification methods (i.e. dactyloscopy, odontology, and molecular genetic analysis). This statement has been issued taking into account the international migration crises related to thousands of deaths worldwide, in which the utility of these traditional means of identification has been sporadic to non-existent. The statement is however not limited to deaths related to the migration crises, as similar problems may occur in fatalities en masse such as in natural disasters and armed conflicts, and on a smaller scale in cases of homeless or otherwise socioeconomically disadvantaged persons. The number of reports on personal identification based on sound anthropological methodology is increasing in the scientific literature. However, more research is needed to develop evidence-based standard operating procedures and statistical frameworks. It remains essential to raise awareness among forensic practitioners, law enforcement, and judiciary professionals on the utility of forensic anthropology in cases where it can provide sufficient information for identification.

Multi-Rater Agreement Using the Adapted Fracture Healing Scale (AFHS) for the Assessment of Tubular Bones on Conventional Radiographs: Preliminary Study.

Better understanding of the timing of fracture healing may help in cases of interpersonal violence but also of personal identification. The intra- and inter-rater agreement for the adapted fracture healing scale (AFHS) assessing the post-traumatic time interval on radiographs were tested. This is a preliminary study, providing essential information on method reliability for upcoming studies using the AFHS. Five raters (two radiologists, a forensic pathologist, an orthopedist, and an anthropologist) were presented with a test in three parts consisting of 85 radiographs (from 30 adults) of fractures of tubular bones in different stages of healing purposefully selected from more than 1500 radiographs. The raters were firstly asked to assess 15 features describing fracture healing as present, absent, or not assessable. Thereafter, the raters were asked to choose from the AFHS a single-stage best representing the observed healing pattern. The intra- and inter-rater agreement were assessed using single-rating, absolute agreement, two-way mixed-effects intra-class correlation (ICC) coefficients. The intra-rater ICC of radiologist 1 ranged from 0.80 to 0.94. The radiologists' inter-rater ICC ranged from 0.68 to 0.74, while it ranged from -0.01 to 0.90 for the other raters. The good to excellent ICC among the radiologists and forensic anthropologist provides good foundation for the use of the AFHS in forensic cases of trauma dating. The poor to good results for the other physicians indicate that using the AFHS requires training in skeletal anatomy and radiology.

Postmortem imaging of perimortem skeletal trauma.

Various imaging modalities, including conventional radiography, computed tomography, magnetic resonance, and surface scanning have been applied in the examination of skeletal injuries in the forensic context. Although still not a substitute for a full medico-legal autopsy or the examination of skeletal remains, imaging is now increasingly used as a complementary tool in the postmortem analysis of perimortem skeletal trauma. Facilitated by the progress in general computational capacity, multimodal imaging has been proposed for comprehensive forensic documentation. A major advantage of these imaging approaches is that stored digital or physical 3D models of skeletal injuries can be reviewed at any time by various experts as well as be presented in court as evidence to clarify potentially complex medical and forensic aspects of the case. Due to constant technical progress in imaging techniques and software, continuous education, training, and sharing of expertise among engineers, computer scientists, and forensic experts, including forensic pathologists, anthropologists, and radiologists needs to be warranted to maintain high-quality expertise in the detection and interpretation of traumatic injuries on postmortem imaging. The technical developments and ever-improving user-friendliness of 3D imaging and modeling techniques present an atttactive alternative to traditional forensic approaches, but as long as the techniques have not been sufficiently tested and validated for forensic trauma analysis, and best practice manuals for forensic practice are lacking for both the technical procedures and method selection, the use of imaging techniques needs to be reevaluated on a case-by-case basis. In addition, ethical, legal, and financial aspects of the use of imaging and 3D modeling for forensic purposes need to be well understood by all parties in legal proceedings.

The Utility of Skeletal and Surgical Features for the Personal Identification Process: A Pilot Study.

This pilot study provides a conceptual framework for the application of the anthropological analysis of skeletal features and surgical interventions for the purpose of identification in cases of unknown deceased individuals with unavailable fingerprint, genetic or odontological antemortem data. The study sample includes 276 individuals with known demographic and clinical information from the Italian CAL Milano Cemetery Skeletal Collection. In the sample, 124 (45%) individuals showed one or more skeletal features that may be potentially individualizing. Of these, 79% showed two and more features, which occurred in a multitude of different combinations. Skeletal findings may provide useful postmortem information that can be compared with antemortem witness statements and clinical imaging. However, more research into the utility of dry bone findings and the availability of comparative material, including imaging, and epidemiological data needs to be undertaken before skeletal features can be implemented into identification protocols and databases.

3D mug shot-3D head models from photogrammetry for forensic identification.

No human face is like another, not even in monozygotic twins, which makes the face one of the most individualizing characteristic. It is for this reason that the human face is commonly used for identification purposes and police officers take portrait photographs of arrested persons, so-called mug shots. The disadvantage of these 2D mug shots is that the perspective, in which they are taken (usually frontal and lateral-right, left or both), cannot be changed after acquisition, thus limiting a potential comparison between a mug shot and surveillance footage or other visual recordings. Documenting a face in 3D would reduce this problem as it allows adjusting the perspective of the face for image comparisons depending on the needs of the investigator. We have developed a 3D mug shot system containing 26 digital single-lens reflex cameras arranged semi-circularly in a 200° arc with a 1.46 m radius around a height-adjustable chair. We generated photogrammetric models of a test person's face captured by the mug shot system using three different focal lengths settings as well as 3D models of the same face with GOM Atos Triple Scan and Artec Space Spider. The 3D models were then analysed regarding the visibility of detailed morphological features in different regions of the face compared to 2D mug shots. Our results showed that our 3D mug shot system with its photogrammetric documentation generates 3D models with comparable surface quality to Artec-generated models, or even better quality, compared to GOM-generated models. The results of the morphological assessment were affected by the focal length and availability of texture information. In conclusion, the 3D mug shot system is a fast and efficient tool to generate 3D models of the face and may be used in addition to 2D photographs for the purpose of visual forensic identification based on images.

The Status of Forensic Anthropology in Europe and South Africa: Results of the 2016 FASE Questionnaire on Forensic Anthropology.

One of the goals of the Forensic Anthropology Society of Europe (FASE) is to map the existing education and practice opportunities in the field of forensic anthropology in order to support the development of the discipline and to optimize the training courses provided by the Society. To address this goal, an online questionnaire was sent to European and South African practitioners of forensic anthropology and related disciplines in 2016. The results of the questionnaire showed that the status and roles of forensic anthropologists vary depending on the national legal systems, education, and employment status of the practitioners. Despite the fact that the expertise of forensic anthropologists has been increasingly requested in a variety of investigations and the spectrum of tasks has become broader, including identification of living persons, specialized education in forensic anthropology is still restricted to a few graduate and postgraduate programs in European countries and to annual FASE courses.

Child trafficking and the European migration crisis: The role of forensic practitioners.

Trafficking in children is one of the worst forms of human rights violation and is categorised as a serious crime. Children at high risk of becoming victims of trafficking are runaways, children with a history of abuse, and migrant children. Internationally, cases of child trafficking are increasing the most in Europe, which is likely the result of the current migration crisis. In crises, preventing and combating human trafficking needs to be prioritized, considering that the aims of humanitarian action include saving lives, easing suffering and preserving human dignity. The involvement of forensic practitioners in investigations of cases of child trafficking mainly concerning the identification of victims may save lives and certainly alleviate suffering of the child victims and their families searching for them. Moreover, by aiding the prosecution process through thorough documentation and expert reporting forensic practitioners may contribute to the protection, rehabilitation and possibly compensation of the child victims, and thus to the restoration of their rights and dignity. So far, forensic practitioners were rarely specifically mentioned as actors in the counter-trafficking efforts in the multitude of policies, regulations, guidelines and recommendations concerning different aspects of child trafficking. This seems surprising considering that the expertise and experience of practitioners from forensic sciences including cyber forensics, document analysis, forensic biology, anthropology, and medicine can be utilised for gathering intelligence in cases of suspected human trafficking, for identifying the victims as well as perpetrators, and for securing evidence for legal proceedings as this paper shows. While this article mainly discusses the role of forensic pathologists and anthropologists, with a specific focus on the identification of child victims of trafficking in the context of the European migration crisis, the notions regarding the contribution of forensic sciences to the counter-trafficking efforts can be adapted to other geographical and sociopolitical contexts.

Personal Identification of Deceased Persons: An Overview of the Current Methods Based on Physical Appearance.

The use of the physical appearance of the deceased has become more important because the available antemortem information for comparisons may consist only of a physical description and photographs. Twenty-one articles dealing with the identification based on the physiognomic features of the human body were selected for review and were divided into four sections: (i) visual recognition, (ii) specific facial/body areas, (iii) biometrics, and (iv) dental superimposition. While opinions about the reliability of the visual recognition differ, the search showed that it has been used in mass disasters, even without testing its objectivity and reliability. Specific facial areas being explored for the identification of dead; however, their practical use is questioned, similarly to soft biometrics. The emerging dental superimposition seems to be the only standardized and successfully applied method for identification so far. More research is needed into a potential use of the individualizing features, considering that postmortem changes and technical difficulties may affect the identification.